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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
17/03/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
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Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
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Thesaurus Nal: |
Mycobacterium abscessus. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
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100 1 $aARAUJO, C. P.
245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource]
260 $c2014
520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
650 $aMycobacterium abscessus
653 $aBovine and bubaline tuberculosis
653 $aNested-PCR system
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aS. FILHO, A. F.
700 1 $aVIDAL, C. E. S.
700 1 $aROXO, E.
700 1 $aNISHIBE, c.
700 1 $aALMEIDA, N. F.
700 1 $aF. JUNIOR, A. A.
700 1 $aSILVA, M. R.
700 1 $aB. NETO, J. D.
700 1 $aCERQUEIRA, V. D.
700 1 $aZUMÁRRAGA, M.
700 1 $aARAUJO, F. R.
773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 9 | |
| 1. |  | OLIVEIRA, C. A.; SILVA, A. O. F.; CERQUEIRA, V. D.; SCOFIELD, A.; ALMEIDA, M. B.; RIET-CORREA, F.; FELIPPE-BAUER, M. L.; RIET-CORREA, G. Allergic dermatitis caused by Culicoides in Texel sheep in the state of Pará, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 301-306, abril 2017. Título em português: Dermatite alérgica causada por Culicoides em ovinos Texel no estado do Pará.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | JUNIOR, H. dos S. B.; TEODORO, V. A. M.; SILVA, M. R.; VICENTINI, N. M.; MIGUEL, E. M.; CERQUEIRA, V. D. Avaliação das mãos de manipuladores da indústria de laticínios. In: CONGRESSO NACIONAL DE LATICÍNIOS, 32., 2019, Juiz de Fora. Resumos... Belo Horizonte: EPAMIG, 2019. p. 79.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | PEIXOTO, P. V.; OLIVEIRA, K. D.; FRANÇA, T. N.; DRIEMEIER, D.; DUARTE, M. D.; BEZERRA JUNIOR, P. S.; CERQUEIRA, V. D.; ARMIÉN, A. G. Experimental and iatrogenic poisoning by sodium selenite in pigs. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 561-569, jun. 2017. Título em inglês: Intoxicação iatrogênica e experimental por selenito de sódio em suínos.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | TRUJILLO, J. G.; TEIXEIRA, M. A. S.; LIMA, A. H. A.; MONTÃO, D. P.; ROSS, T. B.; RIET-CORREA, G.; CERQUEIRA, V. D.; BEZERRA JÚNIOR, P. S. Intoxicação por etanol em bovinos alimentados com bagaço de malte acrescido de levedura de cerveja. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 3, p. 382-386, março 2018 Título em inglês: Ethanol poisoning in cattle fed with malted barley waste with brewer?s yeast.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | TEIXEIRA, M. A. S.; MACHADO, F. M. C.; SARMENTO, N. M. F. P.; OLIVEIRA JÚNIOR, C. A.; RIET-CORREA, G.; CERQUEIRA, V. D.; FRANÇA, T. do N.; BEZERRA JÚNIOR, P. S. Aspectos patológicos e imuno-histoquímicos da polisserosite em búfalos (Bubalus bubalis). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 59-64, janeiro 2018. Título em inglês: Histopathological and immunohistochemical study of the polyserositis in buffalo (Bubalus bubalis).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | PEREIRA, J. D. B.; CERQUEIRA, V. D.; BEZERRA JUNIOR, P. S.; BEZERRA, D. K. O.; ARAUJO, F. R.; DIAS, A. de C. L.; ARAÚJO, C. P.; RIET-CORREA, G. Diagnóstico histopatológico e molecular de lesões sugestivas de tuberculose em búfalos abatidos nos municípios de Macapá e Santana, estado do Amapá. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 11, p. 1198-1204, novembro 2017. Título em inglês: Histopathological and molecular diagnosis of lesions suggestive of tuberculosis in buffaloes slaughtered in the municipalities of Macapá and Santana, Amapá state, Brazil.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Unidades Centrais. |
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| 7. | | BENEDITO JÚNIOR, H. dos S.; TEODORO, V. A. M.; VICENTINI, N. M.; SILVA, M. R.; MIGUEL, E. M.; PAULA, J. C. J. de; COSTA, R. G. B.; SOBRAL, D.; CERQUEIRA, V. D.; GOMES, M. da S. Lista de verificação de Programas de Autocontrole para Indústrias de Laticínios. Indústria de Laticínios, v. 24, n. 140, p. 102-105, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. Plos One, v. 9, n. 3, p. 1-6, 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| 9. | | ARAÚJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA FILHO, A. F.; VIDAL, C. E. S.; VARGAS, A. P. C.; ROXO, E; ROCHA, A. S.; SUFFYS, P. N.; FONSECA JÚNIOR, A. A.; SILVA, M. R.; BARBOSA NETO, J. D.; CERQUEIRA, V. D.; ARAUJO, F. R. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology 45, 2, 2014, P. 633-640., 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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